Statement is true: If you count the names on the outside of the circle (like EcoR I, BamH I, etc.), there are exactly 8 restriction sites shown.

Statement is true: There are 2 sites inside the ampR gene and 2 sites inside the tetR gene. The other 4 sites (EcoR I, Cla I, Hind III, and Pvu II) are outside these areas. Cutting them will not cause a loss of antibiotic resistance.

Statement is true: The diagram clearly shows the rop region at the bottom.

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